The rapidly developing methods of systems biology can help investigators to make inference and predictions from their data that intuition alone would not discern; however, the difficulty lies in the broad knowledge of mathematics andengineering that seems to be required to even evaluate the applicability of the methods to the problem at hand. In Systems Biology, scientists who possess that mathematical knowledge and have applied it effectively to various biological problems contribute methods which reflect their experience, crystallized in a form very similar to typical biological laboratory protocols. In order to best aid researchers, the chapters supply the theory behind the method, its step-by-step implementation, and the pitfalls explained thoroughly and from the practical angle. As a volume of the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Written by leading scientists who apply quantitative systems methodology in their biological research Covers a broad range of methods with a proven record of application to a wide variety of biomedical research problems Step-by-step instructions for implementing computational techniques, much like in a laboratory protocol INDICE: From the contents Introduction: A Practical Guide to the Systems Approach in Biology.- Computational Modeling of Biochemical Networks Using COPASI.- Flux Balance Analysis (FBA): Interrogating Genome-Scale Metabolic Networks.- Modeling Molecular Regulatory Networks with JigCell and PET.- Rule-Based Modeling of Biochemical Systems with BioNetGen.- Ingeneue: A Software Tool to Simulate and Explore Genetic Regulatory Networks.- Microfluidics Technology forSystems Biology Research.- Systems Approach to Therapeutics Design.- Rapid Creation, Monte Carlo Simulation, and Visualization of Realistic 3D Cell Models.- A Cell Architecture Modeling System Based on Quantitative Ultrastructural Characteristics.- Location Proteomics: Systematic Determination of Protein Subcellular Location.- Model-based Global Analysis of Heterogeneous Experimental Data Using gfit.- Multi-Cell Simulations of Development and Disease Using the CompuCell3D Simulation Environment.
- ISBN: 978-1-934115-64-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 470
- Fecha Publicación: 01/03/2009
- Nº Volúmenes: 1
- Idioma: Inglés