Methods of Adipose Tissue Biology Part B: Methods of Adipose Tissue Biology

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale. 1) The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications. 2) Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues. 3) The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels. INDICE: 1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes10. Measurement of Lipolysis11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics12. Methods for Performing Lipidomics in White Adipose Tissue13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis16. Evaluation of Protein Phosphorylation during Adipogenesis

• ISBN: 978-0-12-800280-3
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 344
• Fecha Publicación: 12/04/2014
• Nº Volúmenes: 1
• Idioma: Inglés