This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale. 1) The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications. 2) Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues. 3) The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels. INDICE: 1. Adipocyte-Specific Transgenic and Knockout Models2. Imaging White Adipose Tissue With Confocal Microscopy3. Isolation and Study of Adipocyte Precursors4. Imaging of Adipose Tissue5. Adipose Tissue Angiogenesis Assay6. Quantifying Size and Number of Adipocytes in Adipose Tissue7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion.13. Isolation and Quantitation of Adiponectin Higher Order Complexes14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq15. Analysis and Isolation of Adipocytes by Flow Cytometry16. Flow Cytometry Analyses of Adipose Tissue Macrophages
- ISBN: 978-0-12-411619-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 29/03/2014
- Nº Volúmenes: 1
- Idioma: Inglés