In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on Protein INDICE: TCA Precipitation Coimmunoprecipitation of Proteins from Yeast Coupling Antibody to Cyanogen Bromide-Activated Sepharose Analysis of Protein-Protein Interactions by Coimmunoprecipitation Use and Application of Hydrophobic Interaction Chromatography for Protein Purification Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins Salting out of Proteins Using Ammonium Sulfate Precipitation Using Ion Exchange Chromatography to Purify a Recombinantly Expressed Protein Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins Expression and Purification of Membrane Proteins Explanatory Chapter: Choosing the Right Detergent One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) Coomassie Blue Staining Silver Staining of SDS-polyacrylamide Gel Standard In Vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA Binding Protein Filter Binding Explanatory Chapter: Troubleshooting recombinant protein expression: general Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble Western Blotting using Chemiluminescent Substrates
- ISBN: 978-0-12-420119-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 27/05/2014
- Nº Volúmenes: 1
- Idioma: Inglés