In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on Protein INDICE: Practical steady-state enzyme kinetics Quantification of protein concentration using UV absorbance and Coomassie dyes Preparation of protein samples for mass spectrometry and N-terminal sequencing Lysis of mammalian and Sf9 cells In vivo [35 S]-methionine incorporation Pulse-chase analysis to measure protein degradation Labeling of a protein with fluorophores using Maleimide derivitization Labeling a protein with fluorophores using NHS ester derivitization Protein derivitization-expressed protein ligation Protein biotinylation Purification of His-tagged proteins Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag Immunoaffinity purification of proteins Affinity purification of protein complexes using TAP tags Strep-tagged protein purification Proteolytic affinity tag cleavage Affinity pull-down of proteins using anti-FLAG M2 agarose beads Protein affinity purification using intein/chitin binding protein tags Purification of GST-tagged proteins
- ISBN: 978-0-12-420070-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 15/03/2014
- Nº Volúmenes: 1
- Idioma: Inglés