Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on DNA INDICE: Explanatory chapter: PCR Primer design Explanatory Chapter: How Plasmid Preparation Kits Work Explanatory Chapter: Introducing Exogenous DNA into cells Agarose Gel Electrophoresis Analysis of DNA by Southern Blotting Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE) Molecular Cloning Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® Recombination Cloning Technology and Flp-In T-REx® lines Restrictionless cloning Isolation of plasmid DNA from bacteria Preparation of Genomic DNA from Bacteria Preparation of Genomic DNA from Saccharomyces cerevisiae Isolation of Genomic DNA from Mammalian Cells Sanger Dideoxy Sequencing of DNA Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform Explanatory Chapter: Next Generation Sequencing Generating mammalian stable cell lines by electroporation Transient mammalian cell Transfection with Polyethylenimine (PEI) Site-Directed Mutagenesis PCR-based random mutagenesis Megaprimer Method for Mutagenesis of DNA Explanatory Chapter: Troubleshooting PCR Explanatory Chapter: Quantitative PCR General PCR Colony PCR Chemical Transformation of Yeast Transformation of E. coli via electroporation Transformation of Chemically Competent E. coli
- ISBN: 978-0-12-418687-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 408
- Fecha Publicación: 25/10/2013
- Nº Volúmenes: 1
- Idioma: Inglés