Mass spectrometry of proteins and peptides: methods and protocols

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry.As a result of these rapid developments that have lead to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fieldsfrom microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Comprehensive overview of latest mass spectrometric techniques for protein analysis Step-by-step methods complete with detailed instructions for successful implementation Written by established experts with hands-on experience Extensive, up-to-date references arranged by application INDICE: From the contents Generation and Analysis of Multidimensional Protein Identification Technology Datasets.- Quantitative Peptide and Protein Profiling by Mass Spectrometry.- Label Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach.- Classical Proteomics:Two-dimensional Electrophoresis/MALDI Mass Spectrometry.- The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression.- Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins.- High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation.- Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra.- Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry.- Structure Analysis of N-glycoproteins.- Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins.

• ISBN: 978-1-934115-48-0
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 465
• Fecha Publicación: 01/01/2009
• Nº Volúmenes: 1
• Idioma: Inglés