Zebrafish: methods and protocols

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research. Covers key techniques for zebrafish mutagenesis, transgenesis, and gene expression analysis and imaging Contains practical approaches to chemical and insertional mutagenesis, including frozen storage of zebrafish sperm Includes a range of transgenesis protocols (building transgenes from bacterial artificial chromosomes, transposon- and meganuclease-assisted transgenesis) INDICE: From the contents Part I. Mutagenesis and Mutants. Part II. Transgenesis. Part III. Tissue-Specific Manipulations. Part IV. Analyzing Gene Expression. Part V. Imaging.

• ISBN: 978-1-60327-976-5
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 335
• Fecha Publicación: 01/05/2009
• Nº Volúmenes: 1
• Idioma: Inglés