This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situhybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given. Described here are methods using various cell types and tissues as well asdifferent organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced forsingle cell analysis in human genetics, microbiology, animal and plant sciences. Step-by-step protocols of the cutting edge FISH techniques Easy to follow recipies to ensure successful results Each protocol includes interpretation and evaluation sections of the experimental results as well as troubleshooting tips With brillant colour photos showing the results of fluorescence in situ hybridization on various tissues INDICE: Parts: Molecular cytogenetics, probes and equipment; FISH applications in different tissue preparations; Characterization of marker chromosomes;Characterization of cryptic and epigenetic aberrations; Interphase FISH and characterization of the nuclear architecture; FISH applied in Zoology, Botany and Microbiology; Comparative genomic hybridization (CGH) and array CGH; FISH-internet links.
- ISBN: 978-3-540-70580-2
- Editorial: Springer
- Encuadernacion: Cartoné
- Páginas: 470
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés