Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered ‘riboswitches’ involved in gene expression. The existence of a large array of natural and artificial functional nucleicacids has generated tremendous enthusiasm and new opportunities for molecularscientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems. First in the rapidly emerging field on analytical applications for nucleic acids With interdisciplinary approach, a valuable tool for researchers in chemistry, biology, and engineering One of the first books in the series Integrated Microanalytical Systems, recently renamed Integrated Analytical Systems, edited by R. Potyrailo INDICE: Natural functional nucleic acids: ribozymes, allosteric ribozymes and riboswitches.- Artificial functional nucleic acids: aptamers, ribozymes and deoxyribozymes by in vitro selection.- Overview of functional nucleic acids in analytical applications.- Immobilization of functional nucleic acids for analytical applications.- Fluorescent aptamer sensors.- Fluorescent ribozyme anddeoxyribozyme sensors.- Colorimetric aptamer sensors.- Fluorescent and colorimetric sensors based on functional nucelic acid directed assembly of nanomaterials.- Electrochemical aptamer sensors.- Other aptamer sensors.- Functional nucleic acids based signal amplification systems.- Aptamers in affinity separations: capillary electrophoresis.-Aptamers in affinity separations: stationary separation.- Aptamer microarrays.- The use of aptamers in solid-phase assays.- Functional nucleic acid sensors as screening tools.- Functional nucleic acid-based DNA computation.- Functional nucleic acid-based DNA nanostructures.
- ISBN: 978-0-387-73710-2
- Editorial: Springer
- Encuadernacion: Cartoné
- Páginas: 300
- Fecha Publicación: 01/12/2008
- Nº Volúmenes: 1
- Idioma: Inglés