Gene therapy protocols v. 1 Production and in vivo applications of gene transfer vectors

In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current andemerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. Collects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field INDICE: From the contents Preparation and Quantification of Pseudotyped Retroviral Vector.- Production of Retroviral Vectors for Clinical Use.- Methods for the Production of Helper-Dependent Adenoviral Vectors.- Methods for the Production of First Generation Adenoviral Vectors.- Large Scale Production of Recombinant Adeno-Associated Vectors.- Construction and Production of Recombinant Herpes Simplex Virus Vectors.- Plasmid-based Gene Transfer in Mouse SkeletalMuscle by Electroporation.- Chitosan Nanoparticle–Mediated Gene Transfer.- PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues.- Non-ViralGene Delivery with Cationic Liposome-DNA Complexes.- Applications of Lentiviral Vectors in Non-invasive Molecular Imaging.- Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A.- Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells.- Assessment of CFTR Function After Gene Transfer in Vitro and in Vivo.

• ISBN: 978-1-58829-903-1
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 465
• Fecha Publicación: 01/04/2008
• Nº Volúmenes: 1
• Idioma: Inglés