Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependentsignaling and cell function. Divided into two convenient sections, the volumebegins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Describes cutting-edge lipid analysis and quantification methods Provides a selection of practical approaches to the study of lipid metabolism and recognition in the cellular context Includes reproducible, step-by-step laboratory protocols Chapters written by carefully selected experts inthe field INDICE: From the contents Introduction.- Eicosanoids: generation and detection in mammalian cells.- Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry.- Measurement of Polyphosphoinositides in Cultured Mammalian Cells.- Inositol lipid-dependent functions inSaccharomyces cerevisiae: analysis of phosphatidylinositol phosphates.- Methods for the determination of the mass of nuclear PtdIns4P, PtdIns5P and PtdIns(4,5)P2.- Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin.- Solution and solid state NMR of lipids.- Spatial localisation of PIP2 in phase separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain.- Studying cell-to-cell interactions:an easy method of tethering ligands on artificial membranes.- Ceramide-induced transbilayer (flip-flop) lipid movement in membranes.- Effects of Sterols onthe Development and Ageing of Caenorhabditis elegans.
- ISBN: 978-1-58829-727-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 530
- Fecha Publicación: 01/09/2008
- Nº Volúmenes: 1
- Idioma: Inglés