In Reverse Chemical Genetics: Methods and Protocols, expert authors introducefundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists ofthe necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics Introduces the fundamental resourcesand continues with the most recent molecular technologies Presents applicabletechniques and functional assays utilizing specific small molecules INDICE: From the contents ORFeome Cloning.- Systematic Cloning of an ORFeome Using the Gateway System.- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins.- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study.- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data.- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology.- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources.- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources.- Recombinant Protein Production by a Kaiko-Baculovirus System.- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins.- Application of 2D-DIGE in Cancer Proteomics toward Personalized Medicine.
- ISBN: 978-1-60761-231-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 275
- Fecha Publicación: 01/09/2009
- Nº Volúmenes: 1
- Idioma: Inglés