Drug absorption studies: in situ, in vitro and in silico models

In the last 15 years, a great number of cell- or tissue-based in vitro modelshave been introduced into the biopharmaceutics arena. These models mimic the different biological barriers that a drug has to overcome to finally reach itstarget organ/cell/receptor. These in vitro models have been found very usefulin not only characterising the permeability behaviour of drugs molecules in epithelial and endothelial tissues, but also studying drug delivery systems forimproved delivery and enhanced absorption. Compared to the complex in vivo situation, in vitro models offer a fast, convenient approach with cost advantages most of times. Most importantly, they can be standardised and automatised tobe applicable to the high-throughput screening. Starting at the molecular level of studies, continuing with cell monolayer models (both primary and cell lines) and in situ techniques as a final testing format, the book provides a practical approach to contemporary in vitro techniques for drug absorption studies. Comprehensively summarises the different approaches to assess drug absorption from in vitro models. Gives a review on the current state-of-the-art in pharmaceutical cell biology. Provides readers with helpful protocols for many applications that can be directly used at the laboratory bench INDICE: From the contents Perfused Organ Level/In Situ Techniques.- Modelsfor skin absorption and skin toxicity testing.- Models of the small intestine.- Drug absorption from the colon in situ.- In vivo and ex vivo models for assessing drug delivery across the buccal mucosa.- In situ and ex vivo nasal models for preclinical drug development studies.- The isolated perfused lung for drug absorption studies.- Cellular Level – In Vitro Models of Epithelial and Endothelial Barriers.- In vitro models for investigations of buccal drug permeation and metabolism.- In vitro screening models to assess intestinal drug absorption and metabolism.- In vitro cellular models for nasal drug absorption studies.- In vitro models of the bronchial epithelium.- In vitro models of the alveolar epithelial barrier.- Cell culture models of the corneal epithelium and reconstructed cornea equivalents for in vitro drug absorption studies.

• ISBN: 978-0-387-74900-6
• Editorial: Springer
• Encuadernacion: Cartoné
• Páginas: 540
• Fecha Publicación: 01/02/2008
• Nº Volúmenes: 1
• Idioma: Inglés