The unique chromosome dynamics of meiosis have fascinated scientists for wellover a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. State-of-the-art techniques for analysis of meiotic chromosome dynamics Comprehensive methods for studying meiosis in human and in a large numberof model organisms including fungi, plants, invertebrate animals, zebrafish, and mice Chapters on electron microscopy and conventional and immunofluorescent light microscopy Techniques for enriching and culturing mouse meiotic cells INDICE: From the contents Chromosome Spreading and Immunofluorescence Methods in Saccharomyces cerevisiae.- Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading.- Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae Nuclei.- Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe.- Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis.- Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae.- Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora.- Meiotic Cytogenetics in Coprinus cinereus.- Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes.- Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synaptonemal Complexes From Tomato Microsporocytes.-Cytological Analysis of Meiosis in Caenorhabditis elegans.
- ISBN: 978-1-60761-102-8
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 450
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés