The unique chromosome dynamics of meiosis have fascinated scientists for wellover a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 1, Molecular and Genetic Methods details methods forculturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. State-of-the-art techniques for analysis of meiotic recombination Comprehensive methods for studying meiosis in human and in many commonly used model organisms Chapters on background theory, methodology, and statistical considerations for classical genetic analysis of recombination and crossover interference INDICE: From the contents Interaction of Genetic and Environmental Factorsin Saccharomyces cerevisiae Meiosis: The Devil is in the Details.- OptimizingSporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds.- Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae.- Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae.- Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre/loxP Site-Specific Recombination.- Genetic Analysis of Meiotic recombination in Schizosaccharomyces pombe.- Analysis of Meiotic Recombination in Caenorhabditis elegans.- Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana.- Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial Resolutions.
- ISBN: 978-1-934115-66-4
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 375
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés