Post-translational modification of proteins: tools for functional proteomics

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modification of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology™ series. Comprehensive and cutting edge, Post-translational Modification of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. A fully up-dated and expanded practical guide to protein structure-function relationships. Most comprehensive volume on PTM analysis available today. Contains cutting-edge methods in themost high interest areas of the field. Provides easy-to-follow, readily reproducible protocols and time-saving notes INDICE: From the contents Disulfide Bond Mapping by Cyanylation-Induced Cleavage and Mass Spectrometry. Detection of Post-Translational Modifications byFluorescent Staining of 2D gels. Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods. Analysis of Tyrosine-O-Sulfation. a -Amidated Peptides: Approaches for Analysis. Gamma-Glutamate and b -Hydroxyaspartate in Proteins. Lysine Hydroxylation and Cross-linking of Collagen. Mass Spectrometric Determination of Protein Ubiquitination. Analysis of Sumoylation. Detection and Analysis of Protein ISGylation. Analysis of Methylation, Acetylation and other Modifications in Bacterial Ribosomal Proteins. Analysis of S-Acylation of Proteins. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa. 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern. Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.

• ISBN: 978-1-58829-719-8
• Editorial: Humana Press
• Encuadernacion: Cartoné
• Páginas: 390
• Fecha Publicación: 01/05/2008
• Nº Volúmenes: 1
• Idioma: Inglés