Transgenic wheat, barley and oats: production and characterization protocols

The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocolsfor the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. Comprehensive guide to producing and analysing transgenic wheat, barley and oats Providesbroad introduction and summary covering historical developments and future prospects Includes detailed protocols with troubleshooting tips for biolistic and Agrobacterium transformation Contains chapters on selectable markers and reporter genes in transgenic cereals INDICE: From the contents Transgenic Wheat, Barley and Oats: Production and Characterization.- Selection of Transformed Plants.- Reporter Genes.- Biolistics Transformation of Wheat.- Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos.- Floral Transformation of Wheat.- Highly Efficient Agrobacterium-Mediated Transformation of Wheat via In Planta Inoculation.- Barley Transformation Using Biolistic Techniques.- Barley Transformation Using Agrobacterium-Mediated Techniques.- Transformationof Oats and its Application to Improving Osmotic Stress Tolerance.- Promoter Sequences for Defining Transgene Expression.- Down-Regulation of Gene Expression by RNA-Induced Gene Silencing.- Gene Insertion Patterns and Sites.- Fluorescent In Situ Hybridization to Detect Transgene Integration Into Plant Genomes.- Establishing Substantial Equivalence: Transcriptomics.- Establishing Substantial Equivalence: Proteomics.- Establishing Substantial Equivalence: Metabolomics.

• ISBN: 978-1-58829-961-1
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 350
• Fecha Publicación: 01/10/2008
• Nº Volúmenes: 1
• Idioma: Inglés