Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs). Provides the authority and expertise of leading researchers in myeloid-derived suppressor cellsIncludes comprehensive and detailed protocols to assess the detrimental role of myeloid-derived suppressor cellsPresents the latest release in the Methods in Cell Biology series INDICE: 1. Detection of myeloid-derived suppressor cells by flow cytometryTianju Liu2. Determination of murine myeloid-derived suppressor cells by flow cytometryHiroshi Kitamura3. Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory FunctionsGene Man4. Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral bloodGabriel Cabrera5. CD4+ T cells proliferation assays to analyze Mo-MDSC suppressive functionMarcos López-Hoyos6. Assessment of myeloid-derived suppressor cell differentiation ex vivoEster Blanco7. Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivoDaniel Gallego-Pérez8. Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomesAli Syed Arbab9. Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cellsTing Zhao and Hong Du10. In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor CellsKYU HWAN KWACK and Keith Lough Kirkwood11. Estimating Nitric Oxide (NO) from MDSCs by Griess MethodSaikat Mukherjee12. In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs)Kevin Alicea-Torres
- ISBN: 978-0-443-21807-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 318
- Fecha Publicación: 19/03/2024
- Nº Volúmenes: 1
- Idioma: Inglés