Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts providethe most up-to-date,step-by-step laboratory protocols for examining molecularmachinery and biological functions of exocytosis and endocytosis in vitro andin vivo. Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms. INDICE: From the contents Vesicular Trafficking: Molecular Tools and Targets.- Pharmacological Inhibition of Endocytic Pathways. Is It Specific Enough to be Useful?- In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion.- Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells.- FRAP Analysis of Secretory Granule Lipids andProteins in the Sea Urchin Egg.- A Cell-Free Assay for Endocytosis of E-cadherin.- Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin.- Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation.- Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses.- High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins.- Studying Phagocytosis by Live-Cell Scintillation Proximity Assay.- Transcytosis of Polymeric Immunoglobulin A in Polarized Madin-Darby CanineKidney Cells.
- ISBN: 978-1-58829-865-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 495
- Fecha Publicación: 01/04/2008
- Nº Volúmenes: 1
- Idioma: Inglés