A Laboratory Guide to the Paracellular Channel offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter one provides the foundations of ion transport across cell membrane and tight junction. Chapter two covers the Biochemical approaches for paracellular channels and it's followed by chapter three providing the Biophysical approaches. Chapter four describes and discusses Fixation approaches while chapter 5 describes Tissue preparation. Chapter 6 discusses Light microscopy, while chapter 7 presents the Electron microscopy approach. Chapter 8 covers Transgenic manipulation, including DNA and siRNA, Mutagenesis, Retroviral, Lentiviral, and Adenoviral infection. Chapter 9 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and Crispr. The final chapter discusses the future developments of new approaches for paracellular channel research. Researchers and advanced students in bioscience working on understanding in depth the intercellular channel will benefit from the detailed methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical foundations of paracellular channels, and their connection to disease states. Provides consistent and detailed research methodsCovers various cell, tissue, and animal modelsMethods include step by step guidance from beginner to more sophisticated levels INDICE: 1. Introduction2. Biochemical approaches for paracellular channels3. Biophysical approaches for paracellular channels4. Fixation approaches for paracellular channels5. Tissue preparation approaches for paracellular channels6. Light microscopy for paracellular channels7. Electron microscopy for paracellular channels8. Transgenic manipulation of paracellular channels in cells9. Transgenic manipulation of paracellular channels in mice10. Perspective
- ISBN: 978-0-12-818647-3
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 416
- Fecha Publicación: 02/08/2020
- Nº Volúmenes: 1
- Idioma: Inglés