RNA Modification provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today. Dynamic RNA modifications and their roles in biological regulation are the current frontier of life science researchThis volume of Methods in Enzymology represents up to date knowledge and protocols INDICE: 1. The studies of tRNA modifications and decay Eric Phizicky2. Quantitative mass spectrometry analysis of tRNA modifications in stress response Peter C. Dedon3. tRNA methyltransferase assays Ya-Ming Hou4. SCARLET to detect m6A and mRNA structural changes induced by m6A Tao Pan5. Recognition of RNA modification by innate immune system Mark Helm6. RNA 5-methylcytosine analysis by bisulfite sequencing Matthias Ronald Schaefer7. Transcriptome-wide mapping of m6A Gideon Rechavi8. Target identification of RNA cytosine methyltransferase Bradley R. Cairns9. Incorporation, detection, and recoding of Pseudouridine Yi-Tao Yu and Tao Pan 10. Pseudouridine chemical labeling and profiling Chengqi Yi11. Tanscriptome-wide pseudouridine sequencing Wendy V. Gilbert and Thomas M. Carlile 12. m6A-PA-seq and m6dA-CLIP-seq Chuan He and Kai Chen 13. RNA m6A methyltransferase and demethylase assays Chuan He14. Inosine chemical erasing and ICE-seq Tsutomu Suzuki15. Radical SAM-mediated methylation of ribosomal RNA Danica Fujimori16. RNA isolation and mass spectrometry analysis of RNA modifications Tsutomu Suzuki
- ISBN: 978-0-12-802192-7
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Fecha Publicación: 01/08/2015
- Nº Volúmenes: 1
- Idioma: Inglés