Cryo-electron Tomography: A Journey from Sample Preparation to Data Mining

Cryo-electron Tomography: A Journey from Sample Preparation to Data Mining offers a comprehensive guide to the latest state-of-the-art methods and techniques available to researchers. The book begins with a section on sample preparation, considering a range of different sample types including virus-like particles, viruses, bacteria, organoids and tissues, with some chapters dedicated to specific tools like in situ cellular tomography and correlative techniques. The book then moves on to a section on data mining and specific steps and methods, delivering a complete overview of this advancing technique and providing researchers with the knowledge and tools required for their own investigations. Provides a detailed overview of cryo-ET sample preparation and data mining protocols and approaches in a single resourceIncludes foundational knowledge underpinning the fieldExplores the latest methods, techniques and recent developments in cryo-ETOffers step-by-step guidance to researchers for key proceduresWritten by an international range of experts INDICE: Part I: Sample Preparation 1. VLP (Virus-like particles) 2. Viruses 3. Bacteria 4. n situ cellular tomography 5. Correlative techniques 6. Host pathogen interactions 7. Towards organoids and tissues Part II: Data Mining 1. Sub-tomogram averaging 2. Segmentation 3. Denoising 4. Feature detection 5. Validation

• ISBN: 978-0-443-18829-9
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 360
• Fecha Publicación: 01/12/2024
• Nº Volúmenes: 1
• Idioma: Inglés