Volume 2: Physical Properties and Imaging Methods presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combingDNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume 2: Physical Properties and Imaging Methods provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today. Provides the latest protocols to study the mechanical properties of the nucleus and for imaging down to the molecular level by optical, atomic force, and electron microscopy INDICE: From the contents Physical properties of the nucleus studied by micropipette aspiration.- Mechanical properties of interphase nuclei probed by cellular strain application.- Gene expression in polytene nuclei.- Electron microscope visualization of RNA transcription and processing in Saccharomyces cerevisiae by Miller chromatin spreading.- Combing Genomic DNA for Structural andFunctional Studies.- Using molecular beacons to study dispersal of mRNPs fromthe gene locus.- Mapping cis- and trans- chromatin interaction networks usingChromosome Conformation Capture (3C).- Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope.- Using cells encapsulated in agarose microbeads to analyse nuclear structure and functions.- Investigation of nuclear envelope structure and passive permeability.- Reconstitution of nuclear import in permeabilized cells.- Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System.
- ISBN: 978-1-60327-460-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 510
- Fecha Publicación: 01/09/2008
- Nº Volúmenes: 1
- Idioma: Inglés