Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatinand for examining glycosylation, sumoylation, and ADP-ribosylation of nuclearproteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readilyreproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities. Includes state-of-the-art procedures for isolating and working with nuclei from a wide range of cell types, and procedures for studying their components including pathology-related structures INDICE: From the contents The intranuclear environment.- Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle.- Isolation ofHighly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure.- Working with oocyte nuclei: cytological preparations of active chromatin and nuclear bodies from amphibian germinal vesicles.- Preparation of Arabidopsis Nuclei and Nucleoli.- High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures from Trypanosomes.- Methods for studying the nuclei and chromosomes of dinoflagellates.- Isolation of nucleoli fromEhrlich ascites tumor cells and dynamics of nascent RNA within isolated nucleoli.- Time-lapse microscopy and FRET to analyze the dynamics and interactions of nucleolar proteins in living cells.- Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography.
- ISBN: 978-1-58829-977-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 350
- Fecha Publicación: 01/09/2008
- Nº Volúmenes: 1
- Idioma: Inglés