Once believed to be involved mainly with energetics, including the productionof ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years. In Redox-Mediated Signal Transduction: Methods and Protocols, experienced researchers with backgrounds in both the plant and animal sciences contribute timely methods and techniques that can be used to study this important aspect of biology. Beginning with an overview and methods for measuring compounds that affect redox and the redox state of cells, the book continues with reviews of the use of GFP and its derivatives, methods to study the impact of changing redox on proteins, and methods to study the exact molecular changes that may underlie the mechanisms of action of altering redox, among other subjects. Exciting expanding subject area Written by experienced researchers from both the animal and plant fields Contains some overviews and reviews to help guide understanding INDICE: From the contents The role of redox in signal transduction.- The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence.- Detection and measurement of reactive oxygen intermediates in mitochondria and cells.- Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses: A Review.- Measuring redox changes in vivo in leaves: prospects and technical challenges.- Imaging of intracellular hydrogen peroxide production with HyPer upon stimulation of HeLa cells with EGF.- Tools to investigate ROS sensitive signalling proteins.- Methodsfor preparing crystals of reversibly oxidized proteins: crystallization of protein tyrosine phosphatase 1B as an example.- Methods for the Study of Redox-Mediated Changes in p53 Structure and Function.- Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase.
- ISBN: 978-1-58829-842-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 225
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés