Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes. Covers a subset of enzymes that work in the environment of the biological cell membraneOffers a series of experimental approaches for the isolation and study of enzymes INDICE: Biochemical characterization of RseP, an E. coli S2P proteaseYoshinori AkiyamaSignal Peptidase Enzymology and Substrate Specificity ProfilingRoss E. DalbeyScreening and characterization strategies for Nanobodies targeting membrane proteinsLucía Chávez GutiérrezProbing the activity of eukaryotic rhomboid proteases in vitroMarius LembergStructural Biology of Intramembrane ProteasesYigong ShiModulation of ?-secretase activity: experimental approachesHarald SteinerProbing the structure and function relationships of presenilin by substituted-cysteine accessibility methodTaisuke TomitaA new method to determine the transmembrane conformation of substrates in intramembrane proteolysis by deep-UV Resonance Raman SpectroscopyIban UbarretxenaAn inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membraneSinisa UrbanActivity assays for rhomboid proteasesKvido StrisovskyMechanism and inhibition of rhomboid proteasesKvido StrisovskyReconstitution of Gamma-Secretase in Lipid VesiclesMichael S. WolfeStructural and biochemical analysis of intramembrane prenyltransferases in the UbiA superfamiIyWeikai LiFunctional study of the vitamin K cycle enzymes in live cellsJian-Ke Ti
- ISBN: 978-0-12-812213-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 15/01/2017
- Nº Volúmenes: 1
- Idioma: Inglés