Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes. Covers a subset of enzymes that work in the environment of the biological cell membraneOffers a series of experimental approaches for the isolation and study of enzymes INDICE: A High-Throughput Flurometric Assay for Lipid-Protein BindingWonhwa ChoFluorescence-based in situ quantitative imaging for cellular lipidsWonhwa ChoRecombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2Michael H. Gelb and Gerard Lambeau Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound librariesMichael H. GelbCellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to MembraneChristina C. LeslieSecreted Phospholipase A2 Specificity on Natural Membrane PhospholipidsMakoto MurakamiAnalyses of Calcium-Independent Phospholipase A2beta (iPLA2ß) in Biological SystemsSasanka RamanadhamUsing hydrogen deuterium exchange mass spectrometry to examine protein membrane interactionsJohn E. BurkeLipid-binding C2 domains mediate a diverse class of enzymatic functionsRobert V. StahelinInterfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and ActivitySuren TatulianEnzymology at the Membrane InterfaceDan M. RabenStudying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopyFrédéric CarrièreProbing conformational changes and interfacial recognition site of lipases with surfactants and inhibitorsFrédéric CarrièreMeasuring Phospholipase D enzymatic activity through biochemical and imaging methodsMichael A. FrohmanPld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld's Gef ActivityJulian G. CambroneroPhosphatidic Acid Regulation of PIPKIGuangwei Du

• ISBN: 978-0-12-809419-8
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 12/01/2017
• Nº Volúmenes: 1
• Idioma: Inglés