Cilia: From Mechanisms to Disease Part A

Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. In this volume, different study models are used, such as human cell lines, Mus musculus, Danio rerio, Paramecium caudatum or Chlamydomonas reinhardtii. Besides, protocols to study the genesis, morphology, composition and function of cilia will be addressed. In this book, the ciliary study will be realized by using different techniques such as flow cytometry, time-lapse images of fluorescent ciliary proteins, the use of organoids, the evaluation of the ciliary-GPCR dynamics or genetic modulation by using CRISPR/Cas9. Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary functions Approach to the study of some diseases related to ciliary dysfunction, known as ciliopathies Written in an accessible style by renowned experts in the field INDICE: 1. Protocols to induce and study ciliogenesis Jose Manuel M. Bravo San Pedro2. A Flow Cytometry-Based Approach for the study of primary CiliaFrancesca Ciccolini3. Microscopic observation of human airway ciliary movement using wheat germ agglutininYo Kishimoto4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteinsKoji Ikegami5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture methodYumiko Saito and Yuki Kobayashi6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathiesTatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafishRuxandra Bachman-Gagescu8. Visualizing Multiciliated Cells in the ZebrafishRebecca Ann Wingert9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in ChlamydomonasStephen M. King10. Using Paramecium as a Model for CiliopathiesJudith Van Houten11. Using organoids to study ciliaVincent GUEN12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse modelTang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang

• ISBN: 978-0-443-18586-1
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 312
• Fecha Publicación: 01/04/2023
• Nº Volúmenes: 1
• Idioma: Inglés