The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by stepprotocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Fully up-to-date edition offering cutting edge methods for production of recombinant proteins in plants Provides readily reproducible protocols for researchers in the field of molecular pharming INDICE: From the contents From Neanderthal to Nanobiotech: from plant potions to pharming with plant factories.- Cowpea Mosaic Virus-based systems for the expression of antigens and antibodies in plants.- Transient expression of antibodies in plants using syringe agroinfiltration.- Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants.- Production and localization of recombinant pharmaceuticals in transgenic seeds.- Production of antibody fragments in Arabidopsis seeds.- Production of plantibodies in Nicotiana plants.- Physcomitrella patens: a non-vascular plant for recombinant protein production.- Production of recombinant proteins in suspension-cultured plant cells.- Chloroplast-derived vaccine antigens and biopharmaceuticals: Protocols for expression, purification or oral delivery and functional evaluation.- Protein body induction: a new tool to produce and recover recombinant proteins in plants.
- ISBN: 978-1-58829-978-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 335
- Fecha Publicación: 01/11/2008
- Nº Volúmenes: 1
- Idioma: Inglés