Chemical and Genomic Methods in Nucleic Acid Biology highlights new advances in the field of nucleic acids, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors. Provides the latest information on acids and biology researchesOffers outstanding and original reviews on a range of biological research topicsServes as an indispensable reference for researchers and students alike INDICE: MIE 704: Chemical and genomic methods in nucleic acid biology1. A real-time FRET-based biochemical assay for DNA deaminase enzymology and inhibitionHideki Aihara2. DEER spectroscopy to probe DNA wrapping by protein complexesEdwin Antony3. PAR-dCLIP: Enhancing PhotoActivatable Ribonucleoside analog-enhanced CrossLinking and Immunoprecipitation through capture of bound 5’ terminal RNA fragmentsManuel Ascano4. Site-specific targeting of transgene cDNA insertionKathleen collins5. Simultaneous Profiling of the RNA Targets of Two RNA-Binding Proteins Using TRIBE-STAMPMathieu Flamand6. Ensemble FRET Approach to Directly and Continuously Monitor Protein?DNA InteractionsMark Hedglin7. Utilizing nuclear extracts to characterize protein-DNA interactions at the single-molecule levelBennett Van Houten8. RNA-Guided Protease Activation in CRISPR-CasAilong Ke9. Activity-based profiling of RNA modifying enzymesRalph Kleiner10. Purification of Cas9 and Cas12a for Peptide-Assisted Genome EditingRahul M. Kohli11. Studying the intersection of nucleoside modifications and SARS-CoV-2 RNA-dependent RNA transcription using an in vitro reconstituted systemKristin Koutmou12. Measuring protein stoichiometry with single-molecule imaging in cell-free extractsJoseph Loparo13. Simultaneous probing of transcription, G-quadruplex, and R-loopSua Myong14. A user guide to RT-based mapping of RNA modificationsSigrid Nachtergaele15. AP Endonuclease 1: Biological updates and advances in activity analysisRobert W. Sobol16. Strand-specific PCR-competitive replication and adduct bypass assay for assessing how DNA adducts perturb DNA replication in mammalian cellsYinsheng Wang17. Expression, purification, and biochemical analysis of the RNA-DNA Hybrid Helicase Sen1/SETX from Chaetomium thermophilumR. Scott Williams
- ISBN: 978-0-443-29724-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/11/2024
- Nº Volúmenes: 1
- Idioma: Inglés