Macromolecular crystallography protocols v. 1 Preparation and crystallization of macromolecules

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that haveaccelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. INDICE: 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag. 2. Cloning, production, and purification of proteins for a medium scale structural genomics project. 3.Baculoviral expression of an integral membrane protein for structural studies. 4. Protein Engineering Sonia Longhi, François Ferron, and Marie-Pierre Egloff. 5. Selenomethionyl protein expression. 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule. 7. Screening and optimization methods for non-automated crystallization laboratories. 8. Improving Marginal Crystals. 9. Optimisation Techniques for Automation and High Throughput. 10. Three-dimensional crystallization of membrane proteins. 11. Crystallization of Protein-DNA complexes. 12. Preparation and crystallization of RNA. 13. Crystallisation of RNA-protein complexes Eiji Obayashi.

• ISBN: 978-1-58829-292-6
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 312
• Fecha Publicación: 01/01/2008
• Nº Volúmenes: 1
• Idioma: Inglés