With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sectionsdevoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andnotes highlighting tips on troubleshooting and avoiding known pitfalls. Outlines the current state and future trends for therapeutic antibodies Provides complete guidelines to discover and develop therapeutic antibodies, from antigenproduction to animal studies Presents a modular approach with each part of the book tracing a stage of antibody discovery and preclinical development INDICE: From the contents Therapeutic Antibodies: Current State and FutureTrends – Is a Paradigm Change Coming Soon?- Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery.- Design of a HumanSynthetic Combinatorial Library of Single-chain Antibodies.- Construction of a Human Antibody Domain (VH) Library.- Generation and Selection of Rabbit Antibody Libraries by Phage Display.- Construction of a Large Naïve Human Phage-Displayed Fab Library through One-Step Cloning.- Identification of Target and Function Specific Antibodies for Effective Drug Delivery.- Screening of SpecificInternalization Fab Fragment from Human Naïve Phage Library by Combinational Bio-Panning.- Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41.- Selection of Non-Aggregating VH Binders from Synthetic VH Phage Display Libraries.- Isolation of Full-LengthIgG Antibodies from Combinatorial Libraries Expressed in Escherichia Coli.
- ISBN: 978-1-934115-92-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 490
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés