DNA damage detection in situ, ex vivo, and in vivo: methods and protocols

Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imagingDNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ seriesformat, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, includingmolecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science. The first publication of its kind combines in situ, ex vivo and in vivo techniques Comprehensive evaluation of various types ofDNA damage Detection in live tissues, blood, urine, sperm, cell cultures and tissue sections Detection range from subcellular to the level of a whole live organism Methods for noninvasive detection and monitoring of DNA damage INDICE: In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques.-Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage.- EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level.- In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase.- In Situ Ligation: a Decade and a Half of Experience.-In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections.- 5’OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach.- Detection ofDNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry.- Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis.- Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH).- Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells.- Ultrasound Imaging ofApoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. - Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry.- ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells.- Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes.- Buccal Micronucleus Cytome Assay.- ?-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin.- Immunologic Detection of Benzo(a)pyrene-DNA Adducts.- Non-Invasive Assessment of Oxidatively Damaged DNA: LiquidChromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2’-deoxyguanosine.- Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test.

• ISBN: 978-1-60327-408-1
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 273
• Fecha Publicación: 29/10/2010
• Nº Volúmenes: 1
• Idioma: Inglés