This thesis describes research into the mode of function, inhibition, and evolution of the ribosomal catalytic center, the Peptidyl Transferase Center (PTC)--research that has already led to attempts at improving PTC antibiotics. ThePhD candidate carried out two parallel studies. One using a combination of X-ray crystallography, biochemistry, molecular biology, and theoretical studies to obtain crystal structures of ribosomal particles with antibiotics that target the PTC, revealing the modes of action, resistance, cross-resistance and discrimination between ribosomes of eubacterial pathogens and eukaryotic hosts. In the second parallel study, the candidate synthesized a ribosomal substructure--one that may represent the minimal entity capable of catalyzing peptide bond formation--shedding light on the origin of the ribosome itself. Nominated by the Weizmann Institute, Israel for a Springer Theses Prize. This research has already led to attempts to improve PTC antibiotics. Based on these findings further research into designing a functional construct mimicking a functional proto-ribosome has been initiated. INDICE: Introduction. Methods. Results. Discussion.
- ISBN: 978-3-642-16930-4
- Editorial: Springer Berlin Heidelberg
- Encuadernacion: Cartoné
- Páginas: 80
- Fecha Publicación: 01/12/2010
- Nº Volúmenes: 1
- Idioma: Inglés