The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideasabout hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology,this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approachesfor understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis. Cutting edge approaches for studying plant hormones and their receptors that have been perfected over the last 5 year Provides the basics of the LC-MS and GC-MS methods that are critical to modern hormone research Includes advanced protocols for mutant identification and assaying hormone responses in heterologous systems such as yeast INDICE: Using reverse genetics to develop small knockout collections for specific biological questions.-Visualizing auxin transport routes in Arabidopsis leaf primordia.- Bioinformatic analysis of hormone microarray data.- Measurement of plant hormones by liquid chromatography-mass spectrometry.- Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry.- Pull-down assays for plant hormone research.- Binding assays for brassinosteroid receptors.- Binding assays for abscisic acid receptors.- Cytokinin sensing systems using microorganisms.- Hormone-binding assay using living bacteria expressing eukaryotic receptors.- Discovery of plant microRNAs and short-interfering RNAs by deep parallel sequencing.- The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana.
- ISBN: 978-1-934115-32-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 150
- Fecha Publicación: 01/11/2008
- Nº Volúmenes: 1
- Idioma: Inglés