This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers G protein coupled receptorsContains chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain INDICE: G Protein Coupled Receptors: Techniques for Probing Structure Comformational Ensemble View of G Protein-Coupled Receptors and The Effect of Mutations and Ligand Binding Structural Evolution of G-Protein-Coupled Receptors: A Sequence Space Approach Directed Evolution of G Protein-Coupled Receptors for High Functional Expression and Detergent Stability The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors Structure of ß-Adrenergic Receptors Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G-Protein Coupled Receptors Crystallogenesis of Adenosine A2A Receptor - T4 Lysozyme Fusion Protein: A Practical Route for the Structure Probing GPCR Structure: Adenosine and P2Y Nucleotide Receptors Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis Elegans Expression, Purification and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling Mapping a Ligand Binding Site Using Genetically-Encoded Photoactivatable Crosslinkers Alternative mRNA Splicing of G Protein-Coulped Receptors Functional Residues of the CB1 Cannabinoid Receptor
- ISBN: 978-0-12-391861-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 440
- Fecha Publicación: 15/03/2013
- Nº Volúmenes: 1
- Idioma: Inglés