This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers endosome signalingContains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis. INDICE: Section I. SIGNALING 1. Measurement of insulin proteolysis and assessment of insulin-mediated signaling (events) in (liver) endosomes2. Endosomal sorting and implication for signaling3. TGF-beta in endosomal signaling4. Annexin A6 and endosomal signaling5. PI-3-P in endosomal signaling6.Endosomal signaling and cell migration7. Measuring lipid-ginding effectors in endosomal signaling8. Measuring role for endosomal signaling in tumorigenesis9. Measurement of endosomal signaling in the immune system10. Assessment of the TLR3 pathway in endosomal signaling11. Labeling of platelet-derived growth factor to study its endocytosis and signaling12. Endosomal signaling and oncogenesis13. ROS - Containing endosomal compartments: implications for signaling14. ERBIN in endosomal signaling15. Lipid raft in endosomal signaling16. VEGF-A in endosomal signaling17. Assessment of peptide internalization and endosomal signaling18. Quantitative proteomic analysis of compartmentalized signaling networks19. Death signaling endosomes: sequential activation of Caspase-8, Caspase-9, acid sphingomyelinase and Cathepsin D in internalized TNF-receptosomes20. Deubiquitinases and their emerging roles in cell signaling21. NF-kB-dependent pathway in human keratinocytes22. Endosomal signaling by the G protein coupled PARs23. Investigating signaling consequences of GPCR trafficking in the endocytic pathway24. MICAL-L1 in endosomal signaling
- ISBN: 978-0-12-397925-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 518
- Fecha Publicación: 26/02/2014
- Nº Volúmenes: 1
- Idioma: Inglés