Over the past twenty years, the development of chromatin immunoprecipitation,or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Provides an up-to-date reference volume for chromatin immunoprecipitation assays written by leading researchers in the field Delivers easily accessible and thoroughly tested bench protocols Covers a wide range of biological systems, species and state-of-the-art approaches to analyses INDICE: From the contents The State-of-the-Art of Chromatin Immunoprecipitation.- Characterization and Quality Control of Antibodies Used in ChIP Assays.- The Fast Chromatin Immunoprecipitation Method.- µChIP: Chromatin Immunoprecipitation for Small Cell Numbers.- Fish’n ChIPs: Chromatin Immunoprecipitationin the Zebrafish Embryo.- Epitope Tagging of Endogenous Proteins for Genome-Wide Chromatin Immunoprecipitation Analysis.- Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research.- Serial Analysis of Binding Elements for Transcription Factors.- Modeling and Analysis of ChIP-Chip Experiments.- Use of SNP Arrays for ChIP Assays: Computational Aspects.- DamID: A Methylation-Based Chromatin Profiling Approach.
- ISBN: 978-1-60327-413-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 240
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés