Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore theseexciting new insights, focusing on the study of medically important fungi andCandida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time,the work includes in-depth descriptions of disease models of candidiaisis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification Provides currentmethods for experimentation with Candida albicans Applies Immunological, Molecular, Genetic and Mircobiological approaches Topics include evaluation of vaccines, virulence factor determinants, biofilms, and host cell responses, strain typing and others INDICE: From the contents Isolation of dendritic cells (DC) from human blood for in vitro interaction studies with fungal antigens.- Detection and quantitiaton of anti-fungal SlgA antibodies in body fluids.- Phagocytosis and killing assays for Candida species.- Immunization protocols for use in animal models of candidiasis.- Penetration of antifungal agents through Candida biofilms.-Candidal bioflm analysis in the artificial throat using FISH.- Conditions foroptimal Candida biofilm development in microtiter plates.- Animal models of candidiasis.- Candida albicans gene expression in an in vivo infection model.- In vitro and ex vivo assays of virulence in Candida albicans.- Biotyping of Candida albicans and other fungi by yeast killer toxins sensitivity.- DNA fingerprinting Candida species.- The application of tandem affinity purification to Candida albicans.- Preparation of samples for proteomic analysis of the Candida albicans cell wall.
- ISBN: 978-1-58829-760-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 195
- Fecha Publicación: 01/12/2008
- Nº Volúmenes: 1
- Idioma: Inglés