Proteomic Profiling and Analytical Chemistry: The Crossroads

Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition, helps scientists without a strong background in analytical chemistry understand basic analytical principles and apply them to proteomics profiling. The book bridges the gap between overly specialized courses and books in mass spectrometry, proteomics, and analytical chemistry, helping researchers with an analytical chemistry background to break into the proteomics field. By focusing on practical applications, the book helps readers design better experiments and more easily interpret, analyze, and validate the resulting data. Experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more are covered in detail. The second edition features a new chapter on SWATH-MS, substantial updates to proteomic database search and analytical quantification, an expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics INDICE: Preface1. IntroductionJerzy Silberring and Pawel Ciborowski2. BiomoleculesAriel Burns, Pawel Olszowy and Pawel Ciborowski3. General Strategies for Proteomic Sample PreparationPiotr Suder, Peter Novak, Vladimir Havlicek and Anna Bodzon-Kulakowska4. Protein Extraction and PrecipitationPetr Novak and Vladimir Havlicek5. On-line and Off-line Sample FractionationMarek Smoluch, Przemyslaw Mielczarek, Anna Drabik and Jerzy Silberring6. Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Preparation for Biomarker Discovery in Biological FluidsJayme Wiederin and Pawel Ciborowski7. Gel Electrophoresis Anna Drabik, Anna Bodzon-Kulakowska and Jerzy Silberring8. Quantitative Measurements in Proteomics - Mass SpectrometryAnna Drabik and Jerzy Silberring9. SWATH-MS: Data Acquisition and AnalysisKristin Frederick and Pawel Ciborowski10. Mass Spectrometry-Based Top-Down ProteomicsCory Boone and Jiri Adamec11. Proteomic Database Search and Analytical QuantificationMelinda Wojtkiewicz, Jayme Wiederin and Pawel Ciborowski12. Application of Statistical Analysis to ProteomicsFang Yu, Fang Qiu and Jane Meza13: Analytical ValidationJo-Ellyn McMillan14.Validation in Proteomics and Regulatory AffairsJerzy Silberring, Melinda Wojtkiewicz and Pawel Ciborowski

• ISBN: 978-0-444-63688-1
• Editorial: Elsevier
• Encuadernacion: Rústica
• Páginas: 310
• Fecha Publicación: 31/03/2016
• Nº Volúmenes: 1
• Idioma: Inglés