Immunogenetics: methods and applications in clinical practice

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility andare likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Immunogenetics: Methods and Applicationsin Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field. INDICE: 1. The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome Richard J. N. Allcock 2. HLA Typing by SSO and SSP Methods Heather Dunckley 3. Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity Michael D. Varney, Alison S. L. Castley, Katri Haimila and Päivi Saavalainen 4. HLA Typing Using Bead Based Methods Daniel Trajanoski and Samantha J. Fidler 5. HLA Typing by Direct DNA Sequencing Linda K. Smith 6. Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio CarlaWirtz and David Sayer 7. Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions Holger Nückel, Erick C. Castelli, Philippe Moreau, Crista Ochsenfarth, Peter A. Horn, and Vera Rebmann 8. Molecular Typing of HLA-E Nina Lauterbach, Christina E.M. Voorter, and Marcel G.J. Tilanus 9. Molecular Analysis of Complement Component C4 Gene Copy Number Alison Castley and Olga Patricia Martinez 10. Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5’ Nuclease Allelic Discrimination Mari Malkki and Effie W. Petersdorf 11. High Resolution MICA Genotyping by Sequence-Based Typing (SBT) Yizhou Zou and Peter Stastny 12. Standard Methods for the Management of Immunogenetic Data Pierre-Antoine Gourraud, Jill A. Hollenbach, Thomas Barnetche, Richard M. Single, and Steven J. Mack 13. Analytical Methods for Immunogenetic Population Data Steven J. Mack, Pierre-Antoine Gourraud, Richard M. Single, Glenys Thomson, and Jill A. Hollenbach 14. Analytical Methods for Disease Association Studies with Immunogenetic Data Jill A. Hollenbach, Steven J. Mack, Glenys Thomson, and Pierre-Antoine Gourraud 15. Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View Caner Süsal and Gerhard Opelz 16. Screening for Antibodies Against MICA by Luminex Flow Cytometry Yizhou Zou and Peter Stastny 17. HLA Antibody Detection and Characterizationby Solid Phase Immunoassays - Methods and Pitfalls Andrea A. Zachary, Renato M. Vega, Donna P. Lucas, and Mary S. Leffell 18. Detection and Characterisation of Alloreactive T Cells Mandvi Bharadwaj, Nicole A. Mifsud, and James McCluskey 19. Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells Lloyd J. D’Orsogna, Ellen M.W. van der Meer-Prins, Yvonne M. Zoet, Dave L. Roelen, Ilias I.N. Doxiadis, and Frans H.J. Claas 20. Separation and Cryopreservation of Lymphocytes from Spleenand Lymph Node Gabriella Tassone and Samantha J. Fidler 21. Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC) Samantha J. Fidler 22. The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation Jonathan Downing23. Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System Raja Rajalingam 24. KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP) David Ordóñez, Manuela Moraru, Natalia Gómez-Lozano, Elisa Cisneros, and Carlos Vilches 25. Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing Lihua Hou, Minghua Chen, Noriko Steiner, Kanthi Kariyawasam, Jennifer Ng, and Carolyn K. Hurley 26. An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation Andrea Velardi 27. The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay Dianne De Santis, Bree Foley, Campbell S. Wit, and Frank T. Christiansen 28. Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells David H.McKenna, Diane M. Kadidlo, Sarah Cooley, and Jeffrey S. Miller 29. Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation Eric Spierings and Els Goulmy 30. Donor Registries and Search Strategies Carolyn K. Hurley, Machteld Oudshoorn, and Michelle Setterholm 31. Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance Gurvinder Kaur and Narinder Mehra 32. IMGT® Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS Eltaf Alamyar, Patrice Duroux, Marie-Paule Lefranc, and Véronique Giudicelli 33. IMGT/DomainGapAlign: the IMGT® Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism François Ehrenman and Marie-PauleLefranc 34. Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism Marie-Paule Lefranc and Gérard Lefranc

• ISBN: 978-1-61779-841-2
• Editorial: Humana
• Encuadernacion: Cartoné
• Fecha Publicación: 31/05/2012
• Nº Volúmenes: 1
• Idioma: Inglés