Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more. Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more. Provides up-to-date and extensive protocols for the research on different aspects of celiac disease pathogenesis Includes original figures which help users understand and follow the described protocols Covers research on different aspects of disease, covering a wide range of technologies INDICE: 1. Pepsin-trypsin digested gliadin treatment in intestinal cellsAne Olazagoitia-Garmendia2. Ex vivo gliadin stimulation of intestinal cellsLinda Zhang and Chuan He3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambersOlga Biskou and Amaia Jauregi-Miguel4. Intestinal permeability assessment using lactulose and mannitol in celiac diseaseSilvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, andJose Ramon Bilbao Catala5. In vivo sensitization to gliadin by oral administrationM. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero6. Separation of epithelial and immune cells from biopsy samplesIrati Romero-Garmendia7. Study and isolation of human intestinal dendritic cell and macrophage subsetsElisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo8. Elucidating the role of microbes in celiac disease through gnotobiotic modelingAlberto Caminero, Elena F. Verdu, and Heather J. Galipeau9. In vitro differentiation of macrophages from peripheral blood cells of celiac patientsFederica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challengeConcepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais,N. Lopez-Palacios, and Mercedes Rubio11. Isolation and cryopreservation of peripheral blood mononuclear cellsSara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac diseaseConcepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadinFrancesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi14. Human intestinal organoid models for celiac disease researchValeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri15. Preparation of pepsin trypsin digested gliadin for stimulation experimentsLuis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabsMaialen Sebastian-delaCruz and Ainara Castellanos-Rubio
- ISBN: 978-0-443-19202-9
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 228
- Fecha Publicación: 21/08/2023
- Nº Volúmenes: 1
- Idioma: Inglés