Membrane potential imaging in the nervous system: methods and applications

The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume startswith a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signalsfrom dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach. 1. Outlines methods and procedures in imaging cellular activity in neurons using voltage-sensitive dyes 2. Provides all the necessary material in the form of a practical manual 3. Written by accomplished leaders in the area of voltage-imaging INDICE: Historical overview and general methods of membrane potential imaging.- Design and use of organic voltage sensitive dyes.- Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines.- Combined voltage and calcium imaging and signal calibration.- Use of fast-responding voltage-sensitive dyes for large-scale recording of neuronal spiking activity with single-cell resolution.- Monitoring integrated activity of individual neurons using FRET-based voltage-sensitive dyes.- Monitoring population membrane potential signals from neocortex.- Monitoring populationmembrane potential signals during functional development of neuronal circuitsin vertebrate embryos.- Imaging the dynamics of mammalian neocortical population activity in-vivo.- Imaging the dynamics of neocortical population activityin behaving and freely moving mammals.- Monitoring membrane voltage using two-photon excitation of fluorescent voltage-sensitive dyes.- Random-access multiphoton microscopy for fast three-dimensional Imaging.- Second harmonic imagingof membrane potential.- Genetically encoded protein sensors of membrane potential

• ISBN: 978-1-4419-6557-8
• Editorial: Springer
• Encuadernacion: Cartoné
• Páginas: 400
• Fecha Publicación: 29/10/2010
• Nº Volúmenes: 1
• Idioma: Inglés