Blood samples have consistently proven to be a key source of genetic materialfor a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volumeconcentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Details numerous techniques to perform blood group genotyping, covering prenatal blood grouping frommaternal plasma to multiplex HLA genotyping Presents not only established butalso cutting-edge developed protocols for complex and high-throughput DNA andRNA profiling Covers low- to high-throughput methods for SNP typing such as pyrosequencing or microbeads INDICE: From the contents PCR-Elisa for High-Throughput Blood Group Genotyping.- Single Base Extension in Multiplex Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing.- Multiplex ABO-Genotyping by Minisequencing.- Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays.- Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance.- Multiplex HLA-Typing by Pyrosequencing.- High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach.- Medium- to High-Troughput SNP Genotyping Using VeraCode Microbeads.- The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping.- Nanoliter High-Throughput PCR for DNA and RNA Profiling.- RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis.- RNA Profiling in PeripheralBlood Cells by Fluorescent Differential Display PCR.
- ISBN: 978-1-934115-93-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 305
- Fecha Publicación: 01/11/2008
- Nº Volúmenes: 1
- Idioma: Inglés