Cilia: From Mechanisms to Disease Part B

Cilia: From Mechanisms to Disease Part B, Volume 176 of Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors. Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary functions Approach to the study of some diseases related to ciliary dysfunction, known as ciliopathies Written in an accessible style by renowned experts in the field INDICE: 1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafishMagdalena Cardenas Rodriguez and Iain Drummond2. Human LUHMES and NES cells as models for studying primary cilia in neuronsPeter Swoboda3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locationsRussell J. Ferland4. Single-molecule imaging in the primary ciliumLucien E. Weiss5. Methods to analyze primary cilia in mouse cardiac lesion modelAlfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr.6. Methods to study motile ciliated cell types in a zebrafish brain modeNathalie Jurisch-Yaksi7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome)Diana Valverde8. Modelling ciliopathies in patient-derived primary cellsMarkus Schueler and Kai-Uwe Eckardt9. Analysis of motility and mucociliary function of tracheal epithelial ciliaKoji Ikegami10. High-speed Super-resolution SPEED Microscopy to study Live Primary CiliumWeidong Yang11. Method for siRNA delivery in retina explantsFlorent Poulhès12. Methods to study primary cilia and autophagy in the brainOlatz Pampliega13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamicsChristopher Westlake

• ISBN: 978-0-443-18588-5
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 312
• Fecha Publicación: 01/05/2023
• Nº Volúmenes: 1
• Idioma: Inglés