Microbial carotenoids from bacteria and microalgae: methods and protocols

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used tostudy carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activityare described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic Dunaliella species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in Anabaena sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms. INDICE: 1. Pathways of Carotenoids Biosynthesis in Bacteria and MicroalgaeJ. Paniagua-Michel, Jorge Olmos-Soto, Manuel Acosta Ruiz 2. Selection and Taxonomic Identification of Carotenoids Producing Marine Actinomycetes Francisco Romero, Rosa Isabel Fernández-Chimeno, Juan Luis de la Fuente, and José Luis Barredo 3. Isolation, Characterization and Diversity of Novel Radio-tolerant Carotenoids-producing Bacteria Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 4. A Novel Radio-tolerant Astaxanthin Producing Bacterium Reveals a New Astaxanthin Derivative: Astaxanthin Dirhamnoside Dalal Asker, Tarek S Awad,Teruhiko Beppu, and Kenji Ueda 5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water ­­­­­Dalal Asker, Tarek S Awad, TeruhikoBeppu, and Kenji Ueda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli Hisashi Harada and Norihiko Misawa 7. EngineeringEscherichia coli for Canthaxanthin and Astaxanthin Biosynthesis Qiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaea Patricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16a Rick W. Ye and Kristen Kelly 11. Genetic Modification inBacillus subtilis for Production of C30 Carotenoids Isamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and Pathways Pamela L. Sharpe and Deana J. DiCosimo 14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids Maiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene Hydroxylase Mark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific, Rapid and Sensitive Identification of Carotenogenic Dunaliella Species Jorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing aForeign ?-C-4-carotene Oxygenase Gene Marta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120 Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative Scale José M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial Carotenoids Eva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus Species Laura Pérez-Fons, and Paul D. Fraser 4. A Novel Radio-tolerant Astaxanthin Producing Bacterium Reveals a New Astaxanthin Derivative: Astaxanthin Dirhamnoside Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water ­­­­­Dalal Asker, Tarek S Awad, Teruhiko Beppu, and KenjiUeda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli Hisashi Harada and Norihiko Misawa 7. Engineering Escherichia colifor Canthaxanthin and Astaxanthin Biosynthesis Qiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaea Patricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278 Eric Giraud, andAndré Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain16a Rick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 Carotenoids Isamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and Pathways Pamela L. Sharpe and Deana J. DiCosimo 14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids Maiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene Hydroxylase Mark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific, Rapid and Sensitive Identification of Carotenogenic Dunaliella Species Jorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign ?-C-4-carotene Oxygenase Gene Marta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120 Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative Scale José M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial Carotenoids Eva Eylert, Adelbert Bacherand Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus Species Laura Pérez-Fons, and Paul D. Fraser 5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water ­­­­­Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli Hisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis Qiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaea Patricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278 Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16a Rick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 Carotenoids Isamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification ofChromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and Pathways Pamela L. Sharpe and Deana J. DiCosimo 14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids MaikoFurubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene Hydroxylase Mark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific, Rapid and Sensitive Identification of Carotenogenic Dunaliella Species Jorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign ?-C-4-carotene Oxygenase Gene Marta Vila, AuroraGalván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120 Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative Scale José M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial Carotenoids Eva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis ofDiapocarotenoids Found in Pigmented Bacillus Species Laura Pérez-Fons, and Paul D. Fraser 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli Hisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis Qiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaea Patricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278 Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp.Strain 16a Rick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 Carotenoids Isamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization ofCarotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and Pathways Pamela L. Sharpe and Deana J. DiCosimo 14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids Maiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene Hydroxylase Mark A. Scaife, Cynthia A. Ma, Phillip C. Wright,and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific, R

• ISBN: 978-1-61779-878-8
• Editorial: Humana
• Encuadernacion: Rústica
• Fecha Publicación: 30/06/2012
• Nº Volúmenes: 1
• Idioma: Inglés