RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, RNAi: Design and Applications enablesa researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field. Step-by-step instructions in a laboratory bench protocol format. Detailed lists of the necessary equipment and reagents. Tips on troubleshooting from actual experience of the experts. Example data set in most chapters. Variety of organisms and applications INDICE: From the contents Principles of Dicer Substrate (D-siRNA) Design and Function. Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes. In vitro RNA Cleavage Assay for Argonaute-Family Proteins. Identifying siRNA-Induced Off-Targets by Microarray Analysis. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis. Nasal Delivery of siRNA. RNA Interference as a Genetic Tool in Trypanosomes. Lentivirus-Mediated RNA Interference in Mammalian Neurons. Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica. Use of RNAi in C. elegans. Applicationof siRNA against SARS in the Rhesus Macaque Model. siRNA and shRNA as Anticancer Agents in a Cervical Cancer. Identification and Expression Analysis of Small RNAs During Development. Screening and Identification of Virus-Encoded RNA Silencing Suppressors.
- ISBN: 978-1-58829-874-4
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 290
- Fecha Publicación: 01/05/2008
- Nº Volúmenes: 1
- Idioma: Inglés