Chemoselective and Bioorthogonal Ligation Reactions: Concepts and Applications

This timely, one–stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others. INDICE: PREFACE. PART 1: CONCEPTS. A Brief Introduction to Traditional Bioconjugate Chemistry. [3+2] Dipolar Cycloadditions in Bioconjugation. Diels–Alder and Inverse Diels–Alder Reactions. The Staudinger Ligation. Thiol–Ene Chemistry. Ligand–Directed Tosyl and Acyl Imidazole Chemistry. Bioorthogonal Labeling of Cellular Proteins by Enzymatic and Related Mechanisms. Metal–Mediated Bioconjugation. PART 2: APPLICATIONS. Protein and Antibody Labeling. Activity–Based Protein Profiling. Nucleic Acid Labelling, Ligation, and Modification. Chemoselective Reactions for Glycan Labeling. Chemoselective Attachment of Lipids to Proteins. In Vivo Applications of Bioorthogonal Chemistries. Immobilization of Biomolecular Probes for Arrays and Assay: Critical Aspects of Biointerfaces. Chemical Ligations in the Design of Hydrogel Materials. Nanoparticle Bioconjugates: Materials that Benefit from Chemoselective and Bioorthogonal Ligation Chemistries. Application of Engineered Viral Nanoparticles in Materials and Medicine.

• ISBN: 978-3-527-33436-0
• Editorial: Wiley VCH
• Encuadernacion: Cartoné
• Páginas: 768
• Fecha Publicación: 19/04/2017
• Nº Volúmenes: 1
• Idioma: Inglés